Contemplation Crossing

Goal

Crossing the Contemplation vine with a renamed clone of itself, and watching for signs of a genome subtraction mutation.

Long-term goal is to create a very short vine genome, with a variety genes, to be used in testing drops of Mutagen and mapping their effects.

Starting Point

Contemplation is a 20 gene vine with genomes of KK, QQ, wildS, and a Hue of Light Red

KRYYRGYROGGGGGRYYROK

Peacefulness#19 is a mutation of Contemplation, with gene #3 or #4 deleted.
Its genome yields tends of KK and Q with a Hue of Light Red.

KRYRGYROGGGGGRYYROK

Further crossbreeding of this vine is on the Peacefulness19 page

Results Summary

• Peacefulness #18 is KK QQ wildS and Light Red Hue. 5/52 chance of a gene deleted
• Peacefulness #19 is KK Q wildS.
  • There are seven possibilities of genes that could be deleted to give this result, resulting in 5 unique genomes.
  • First revelation solvent application (Minu's) showed inconclusive results. Still 5 genome possibilities.
  • Second Minu's application shows that either gene #3 or gene #4 was deleted. Genome of Peacefulness #19 is:
    • KRYRGYROGGGGGRYYROK

Mutation Chances

• 50% no change. 36/72
• 25% gene added. 18/72
• 25% gene deleted. 18/72

Watching for the following changes when tending:

• K genome instead of KK, seen in 7/72 children
  • gene deleted in 5/7
  • gene added in 2/7
• Q genome instead of QQ seen in 12/72 children
  • gene deleted in 8/12
  • gene added in 4/12
• S and Q genome instead of wildS and QQ, seen in 4/72 children
  • gene added in 4/4.

Contemplation Hue is Light Red when examining harvested grapes

• Hue changes to White in 4/72 children
  • gene deleted in 3/4, one tends as KK QQ, two tend as K QQ
  • gene added in 1/4, tends as KK QQ

Peacefulness#18

• tend 1: matches on QQ and KK. Can't tell on wildS/S, but all possibilities with S are single Q
• tend 2: same as tend 1
• tend 3: matches on KK and wildS. Can't tell on QQ
• tend 4: same as tend 3
• tend 5: matches on wildS. Can't tell on QQ and KK
• Hue is Light Red.

Peacefulness#19

Vine is 19 genes long, KRYRGYROGGGGGRYYROK

• tend 1: Matches on KK and wildS. Does not match on QQ. Could be Q (negative tend).
  • 12 children have Q instead of QQ
    • 4/12 are longer and are Q KK S
    • 1/12 is shorter and is Q K wildS
    • 7/12 are shorter and are Q KK wildS
    • 12/12 have Light Red Hue
• tends 2, 4, 5: Matches Q KK wildS
• tend 3: Matches Q. Can't tell on KK and wildS
• All mutations with Q KK wildS have the same hue. Harvesting and checking the hue is not necessary.