Peacefulness19

Goal

I should have named this page "Contemplation 19" but I wasn't expecting to become so confused about all these crosses!

Continued crossbreeding of the Contemplation vine with 19 genes. Working toward children which are:

• short enough to be mapped with a single revelation solvent
• have no Y genes, to be paired with a vine that has all Y genes.

Tends

There are 68 possible children, created by crossing a Contemplation vine with 19 genes.

• 34/68 are genetically identical to the parent, Q KK, Light Red Hue
• 17/68 are mutations with one gene duplicated at the splice point
• 17/68 are mutations with one gene deleted at the splice point

• 53/68 will have tends of Q KK, and have Light Red Hue.
  • 34/53 ARE the same as the parent.
  • 19/53 are mutations with the same tends and hue as the parent.
• 15/68 will be mutations that can be detected through tends or hue (see below)

• 1/15 will have tends of QQ KK, and Light Red Hue (duplication)
• 6/15 will have tends of wildQ and Light Red Hue
  • 3/6 will have tends of wildQ KK, and Light Red Hue (all deletions)
  • 2/6 will have tends of wildQ KK S, and Light Red Hue (both duplication)
  • 1/6 will have tends of wildQ K, and Light Red Hue (deletion)
• 4/15 will have a White Hue
  • 2/4 will have tends of Q KK, and White Hue (one deletion, one duplication)
  • 2/4 will have tends of Q K, and White Hue (both deletions)
• 4/15 will have tends of Q K and Light Red Hue (two duplications, two deletions)

Current Strategy

Repeated crossing of Peacefulness#19 with a renamed clone of itself (Peace19) which has the genome of:

• KRYRGYROGGGGGRYYROK
• This vine has one Y removed from Contemplation, and is 19 genes long. There are four Y genes remaining.
• The hue is Light Red, and the starting genome tends as Q KK wildS

Outcomes

Peacefulness#21

has a 8/53 (about 15%) chance of a deleted gene
considering molecular balance

• Hue is Light Red
• tend 1 matches KK and wildS. Q is possible.
• tend 2 matches Q KK wildS

• 34/68 results have no change to the genome. The tends of this vine match the original
• 34/68 results have a mutation
  • 19/34 mutations also have the same tends as the original
    • 8/19 have a deleted gene. (5 are -G, 2 are -R, 1 is -Y)

considering for molecular balance

Peacefulness#22

has a 8/53 (about 15%) chance of a deleted gene
considering molecular balance

• Hue is Light Red
• tend 1: matches Q KK, cannot tell with wildS
• tend 2: matches Q KK, cannot tell with wildS
• only one cross does not have wildS, and it is a longer genome

Peacefulness#23

has a 8/53 (about 15%) chance of a deleted gene
considering molecular balance

• Hue is Light Red
• tend 1, 2: Matches Q KK, cannot tell with wildS
• tend 3, 4: matches Q KK wildS

Peacefulness#24

This vine has a duplication of the R gene.
Molecular balance indicates that this vine is longer than its parent.

• tend1: Matches Q. Does not match KK. Cannot tell with wildS (not a positive tend)
  • Other possibility is K. KK is a positive tend, K is a negative tend. First tend did not show a positive tend.
• Should be Q K. Hue not yet known
  • 6 possibilities yield Q K
    • 4/6 are Q K H
      • 2/4 are an R gene duplication
      • 2/4 are a gene deletion
    • 2/6 are Q K wildH.
      • both are gene deletion
• tends 2, 3: match Q K wildS
• Hue is Light Red. Not wildH

Peacefulness#28

• tend 1, 2: Q KK wildS
• Hue is light red